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opera phenix plus high-content screening system  (Revvity)


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    Structured Review

    Revvity opera phenix plus high-content screening system
    Opera Phenix Plus High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opera+phenix/Opera+Phenix+Plus+high-content+screening+system/custom%40hh14001000%4042089082
    Average 96 stars, based on 2516 article reviews
    opera phenix plus high-content screening system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Imaging:

    Article Title: Mosaic gastruloids reveal a temporal restriction for developmental cell competition.
    Article Snippet: Microscopy was conducted using a Zeiss LSM980 confocal laser scanning (Airyscan) microscope. .. Alternatively, for high-throughput imaging, immunostained gastruloids were transferred in PBS−/− into 96-well PhenoPlates (Revvity/ PerkinElmer, 6055302) with up to 12 gastruloids per well, followed by automated Z-stack tile imaging on an Opera Phenix (Revvity/ PerkinElmer) in spinning disk configuration. .. The following antibodies were used for indirect immunofluorescence: mouse anti-p53 (1:4,000, clone 1C12; Cell Signaling, 2524S), rabbit anti-phospho-histone 3 (1:1,600, clone D2C8, Cell Signaling, 3377), rabbit anti-cleaved caspase 3 (1:5,000, clone 5A1E, Cell Signaling, 9664), rabbit anti-YAP (1:200, clone D8H1X, Cell Signaling, 14074), goat anti-E-Cadherin (1:1,000, R&D, AF648), rabbit anti-N-Cadherin (1:200, Abcam, ab18203), rabbit anti-FoxA2 (1:400, clone D56D6, Cell Signaling, 8186), goat anti-Tbx6 (1:200, R&D, AF4744), goat anti-Brachyury (1:100, R&D, AF2085), rabbit anti-Sox2 (1:200, Abcam, ab92494), goat anti-Otx2 (1:200, R&D, AF1979), rabbit anti-Sox3 (1:300, Invitrogen, PA5-35983), rat anti-Nanog (1:250, Invitrogen, 14-5761-80), goat anti-Pecam1 Alexa Fluor 488-conj. (1:400, R&D, FAB3628G-025), mouse anti-SSEA-1 Alexa Fluor 405-conj. (1:400, R&D, FAB2155V-100UG), donkey anti-Goat Alexa Fluor 488-conj. (1:500, Invitrogen, A-11055), goat anti-rabbit Alexa Fluor 488-conj. (1:500, Invitrogen, CA-11034), goat anti-rat Alexa Fluor 488-conj. (1:500, Invitrogen, A-11006) and donkey anti-mouse Alexa Fluor 488-conj. (1:500, Invitrogen, A-32766).

    Article Title: Deep learning-based differential diagnosis of major depression and bipolar disorder using microglia-cellular sensors and patient-derived small extracellular vesicles
    Article Snippet: .. Moreover, the growing availability of automated high-content imaging platforms, such as Opera Phenix (Revvity/PerkinElmer) and ImageXpress Micro Confocal (Molecular Devices), provides feasible routes for further standardization and high-throughput scaling. ..

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules
    Article Snippet: After incubation with primary antibodies, anti-βIII-tubulin antibody (1:2,000, Cell Signaling Technology), overnight at 4°C, cells were washed three times with PBS and incubated with appropriate secondary antibodies for 1 h at room temperature. .. Stained cortical neurons were imaged using Opera Phenix (PerkinElmer), and neurite outgrowth rate was measured by Harmony High Content Imaging (PerkinElmer). ..

    Article Title: Deep learning-based differential diagnosis of major depression and bipolar disorder using microglia-cellular sensors and patient-derived small extracellular vesicles.
    Article Snippet: .. Moreover, the growing availability of automated high-content imaging platforms, such as Opera Phenix (Revvity/PerkinElmer) and ImageXpress Micro Confocal (Molecular Devices), provides feasible routes for further standardization and high-throughput scaling. ..

    Flow Cytometry:

    Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses
    Article Snippet: .. Cells were then fixed with 4% paraformaldehyde (PFA), and plates were acquired either using the NovoCyte apparatus (Agilent) for flow cytometry or the Opera Phenix (Revvity) for microscopy. .. Flow cytometry data were then analyzed using the NovoExpress software (Agilent), while fluorescence microscopy data were analyzed with Harmony software (Revvity).

    Microscopy:

    Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses
    Article Snippet: .. Cells were then fixed with 4% paraformaldehyde (PFA), and plates were acquired either using the NovoCyte apparatus (Agilent) for flow cytometry or the Opera Phenix (Revvity) for microscopy. .. Flow cytometry data were then analyzed using the NovoExpress software (Agilent), while fluorescence microscopy data were analyzed with Harmony software (Revvity).

    Staining:

    Article Title: Phenotypic screening of human iPSC-derived neurons identifies thienopyridones as neuritogenic small molecules
    Article Snippet: After incubation with primary antibodies, anti-βIII-tubulin antibody (1:2,000, Cell Signaling Technology), overnight at 4°C, cells were washed three times with PBS and incubated with appropriate secondary antibodies for 1 h at room temperature. .. Stained cortical neurons were imaged using Opera Phenix (PerkinElmer), and neurite outgrowth rate was measured by Harmony High Content Imaging (PerkinElmer). ..

    Software:

    Article Title: Modulators of beta catenin and uses thereof
    Article Snippet: Twenty-four hours after compound treatment, cells were fixed with the addition of 2 μL of 12% formaldehyde (Sigma, 1.04003) at room temperature for 15 minutes, permeabilized in 0.1% Triton X-100 (Sigma, 93443) in PBS for 15 minutes, blocked in 0.2% gelatin from cold water fish (Sigma, G7765) in PBS for 1 hour, and then stained with a 1:1000 dilution of the phospho beta catenin antibody (Invitrogen, 703638) in 0.2% gelatin overnight at 4° C. followed by incubation with a 1:10000 dilution of Hoechst (Thermo, 1-13570) and with a 1:1000 dilution of secondary antibody (Thermo, A11070) for 1 hour at room temperature. .. Plates were then imaged (Opera Phenix, Perkin Elmer), and analyzed with the Harmony software quantitating the fraction of cells with bright phospho beta catenin depots (see FIG. 1). ..



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    Revvity opera phenix plus high-content screening system
    Opera Phenix Plus High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opera+phenix/Opera+Phenix+Plus+high-content+screening+system/custom%40hh14001000%4042089082
    Average 96 stars, based on 1 article reviews
    opera phenix plus high-content screening system - by Bioz Stars, 2026-09
    96/100 stars
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